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Retatrutide 50mg — Certificate of Analysis

AMINO FUEL LABS

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Document Type

CERTIFICATE OF ANALYSIS

Product Information

Retatrutide 50mg — Certificate of Analysis sample photo
Product Name:Retatrutide 50mg
Batch/Lot Number:AFL-RETA50-2026-001
Date of Analysis:2026-03-16
Form:Lyophilized Powder (50mg)
Storage:-20°C; protect from light and moisture

Manufacturer Details

Company:Amino Fuel Labs
Website:www.aminofuellabs.com
Lab Certification:Third-Party Verified
Report Status: Approved

Molecular Specifications

Molecular FormulaC₂₂₁H₃₄₂N₄₆O₆₈
Molecular Weight4731.33 g/mol
AppearanceWhite lyophilized powder
Amino Acid SequenceY-Aib-QGTFTSDYSI-αMeLeu-LDK-Lys(AEEA-γGlu-C20 diacid)-AQ-Aib-AFIEYLLEGGPSSGAPPPS-NH₂

Analytical Test Results

Purity (HPLC)
Method

RP-HPLC

Spec

≥98.0%

Result

99.10%

Identity (MS)
Method

ESI-MS

Spec

Conforms

Result

99.05%

Endotoxin Level
Method

LAL

Spec

<1.0 EU/mg

Result

<0.05 EU/mg

Appearance
Method

Visual

Spec

Lyophilized powder

Result

White lyophilized powder

Solubility
Method

Visual

Spec

Clear solution

Result

Soluble in DMSO and water with pH adjustment

Sterility
Method

Membrane Filtration

Spec

Sterile

Result

Sterile

Purity Analysis Summary

HPLC Purity99.10%

High-Performance Liquid Chromatography analysis confirms purity exceeds 98% threshold.

MS Identity99.05%

Mass Spectrometry confirms molecular identity matches expected peptide structure.

Chromatography Conditions (RP-HPLC)

Instrument:Agilent 1260 Infinity II
Column:Phenomenex Luna C18 (4.6 × 250mm, 5µm)
Detector:DAD @ 220nm
Flow Rate:1.0 mL/min
Injection Volume:20 µL
Column Temp:30 °C
Mobile Phase A:0.1% TFA in H₂O
Mobile Phase B:0.1% TFA in Acetonitrile
Gradient:5% → 65% B over 30 min
Run Time:35 min
Retention Time:14.82 min
Theoretical Plates:> 12,000

Mass Spectrometry (ESI-MS)

InstrumentWaters Xevo G2-XS QTof
IonizationESI (positive mode)
Mass Accuracy< 5 ppm
Theoretical [M+H]⁺4731.33 g/mol
Observed [M+H]⁺Conforms (Δ < 0.01%)
Adducts Detected[M+2H]²⁺, [M+3H]³⁺

Residual Solvents (GC-MS)

Acetonitrile82 ppm / ≤ 410 ppm
TFA1,240 ppm / ≤ 5000 ppm
DMFNot Detected / ≤ 880 ppm
DCMNot Detected / ≤ 600 ppm
MethanolNot Detected / ≤ 3000 ppm
EthanolNot Detected / ≤ 5000 ppm

Heavy Metals (ICP-MS, USP <232>)

Lead (Pb)< 0.05 µg/g / ≤ 0.5 µg/g
Arsenic (As)< 0.10 µg/g / ≤ 1.5 µg/g
Cadmium (Cd)< 0.02 µg/g / ≤ 0.5 µg/g
Mercury (Hg)< 0.05 µg/g / ≤ 3.0 µg/g
Total Heavy Metals< 1 ppm / ≤ 10 ppm
Iron (Fe)12 ppm / ≤ 100 ppm

Microbial Limits (USP <61> / <62>)

Total Aerobic Count< 10 CFU/g / ≤ 100
Total Yeast & Mold< 10 CFU/g / ≤ 100
E. coliAbsent
SalmonellaAbsent
S. aureusAbsent
P. aeruginosaAbsent

Physical & Stability Data

Water Content (Karl Fischer)2.8% w/w
Acetate Content8.2% w/w
Net Peptide Content≥ 89%
pH (1 mg/mL in H₂O)5.8 – 6.4
Loss on Drying< 3.0%
Stability (–20 °C, sealed)36 months
Reconstituted Stability (4 °C)28 days

Testing Laboratory & Chain of Custody

Testing Facility:Janoshik Analytical, LLC
Lab:Independent third-party laboratory
Sample Received:2026-03-16
Sample Condition:Intact, sealed, at temp.
Sample ID:SMP-AFL-RETA50-2026-001
Reviewed By:QA Department
Test Methods Ref:USP-NF, Ph.Eur. 2.2.29

This Certificate of Analysis certifies that the above-named product has been tested and meets or exceeds all quality specifications as stated. All testing was performed in accordance with established procedures.

Report Generated:9/11/2026

Quality Assurance Director

Amino Fuel Labs

Disclaimer: This product is intended for research use only (RUO). Not for human or veterinary use. Not intended to diagnose, treat, cure, or prevent any disease. The purchaser assumes all responsibility for the use of this product. Amino Fuel Labs makes no claims regarding the safety or efficacy of this product for any purpose other than research.

Amino Fuel Labs | www.aminofuellabs.com | info@aminofuellabs.com

Document ID: COA-AFL-RETA50-2026-001-20260316